Development of an intelligent self-tuning PID controller

Advances in Instrumentation, Proceedings47pt 21101-1111AVIN

Multi-axial spine biomechanical testing system with speckle displacement instrumentation

10.1115/1.1493803Journal of Biomechanical Engineering1244471-477JBEN

A benchtop plant for evaluation of flexible structure control methodologies

IECON Proceedings (Industrial Electronics Conference)21416-1421IEPR

Orthogonal array design as a chemometric method for the optimization of analytical procedures part 3.* five-level design and its application in a polarographic reaction system for selenium determination

The Analyst1202273-27

Orthogonal array design as a chemometric method for the optimization of analytical procedures part 4.* mixed-level design and its application to the high-performance liquid chromatographic determination of polycyclic aromatic hydrocarbons

The Analyst1202281-28

Biofunctionalized indigo-nanoparticles as biolabels for the generation of precipitated visible signal in immunodipsticks

A novel class of organic nanoparticles as biolabels that can generate an instant visible signal was applied to immunodipsticks. A new principle for signal generation based on hydrolysis of colourless signal precursor molecules to produce coloured signal molecules followed by signal precipitation and localization was demonstrated. The nanoparticle biolabels were applied to sandwich immunoassays for the detection of mouse immunoglobulin G (M IgG). In the presence of M IgG, a nanoparticle-immunocomplex was formed and bound on the test zone immobilized with goat anti M IgG (Gt α M IgG). A blue line was developed on the test zone upon the addition of a signal developing reagent. An optical signal could be simply assessed using naked eyes or quantified using a reading device. The lowest visible signal that could be observed using naked eyes was found to be 1.25μgL-1M IgG. The nanoparticle biolabel also showed a better sensitivity (signal-to-noise ratio) compared with the conventional colloidal gold biolabel. This novel class of organic nanoparticles offers an alternative biolabel system for the development of point-of-care immunodipsticks. © 2010 Elsevier B.V

Vpliv razdalje sajenja na pridelek hrušk (Pyrus communis L.) sorte \u27Viljamovka\u27

A method for the rapid diagnosis of early dengue virus (DENV) infection is highly needed. Here, a prototype reverse transcriptionrecombinase polymerase amplification (RT-RPA) assay was developed. The assay detected DENV RNA in <20 min without the need for thermocycling amplification. The assay enabled the detection of as few as 10 copies of DENV RNA. The designed RT-RPA primers and exo probe detected the DENV genome of at least 12 genotypes of DENV circulating globally without crossreacting with other arboviruses. We assessed the diagnostic performance of the RT-RPA assay for the detection of DENV RNA in 203 serum samples of patients with clinically suspected dengue. The sera were simultaneously tested for DENV using a reverse transcription-loop-mediated isothermal amplification (RT-LAMP) assay, quantitative RT-PCR (qRT-PCR), and IgM- and IgGcapture enzyme-linked immunosorbent assays (ELISA). Acute DENV infection was confirmed in 130 samples and 61 of the samples (46.9%) were classified as viremic with qRT-PCR. The RT-RPA assay showed good concordance (κ of ≥0.723) with the RTLAMP and qRT-PCR assays in detecting the dengue viremic samples. When used in combination with ELISA, both the RT-RPA and RT-LAMP assays increased the detection of acute DENV infection to ≥95.7% (≥45/47) in samples obtained within 5 days of illness. The results from the study suggest that the RT-RPA assay is the most rapid molecular diagnostic tool available for the detection of DENV. Hence, it is possible to use the RT-RPA assay in a laboratory to complement routine serology testing for dengue